Generator

Part:BBa_K624062:Design

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

pYMB essentials + RBS(trunc.) + Inv


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1929
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 2546
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Invasin: PCR amplification from the genomic DNA of Yersinia enterocolitica

References

pYMB:

Y. Okamura, et al. Design and Application of a new cryptic plasmid-based shuttle vector for Magnetospirillum magneticum. Appl. Environ. Microbiol., 69 (2003), pp. 4274–4277.


Invasin:

Isberg RR, Leong JM: Multiple β1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells. Cell 1990, 60(5):861-871.